It will be the first meeting between the Asian heavyweights since the Asia Cup tournament held Bangladesh in March this year where India won by six wickets.
India won a thriller against Pakistan by five runs to emerge champions in the inaugural World Twenty20 tournament held in South Africa in 2007.
The Indo-Pak warm-up match will be preceded by an Indo-Lanka clash at the P. Sara Oval on September 15.
Pakistan's second warm-up match will be against defending champions England on September 19 at the P. Sara Oval.
It could be the only occasion these two sides meet during the World T20 extravaganza since India have been drawn in Group A with defending champions England while Pakistan are Group D favourites.
However, depending on their standings in the Super Eights, there is still a possibility of a mouth-watering battle between the two rivals in the knockout stages.
Hosts Sri Lanka's will face West Indies in their opening warm-up match on September 13 at NCC grounds.
A total of 12 warm-up matches will be played ahead of the group stages of the September 18 to October 7 event.
